Translational Bioinformatics of HIV Peptide 2.0: From Dry To Wet Bench
نویسندگان
چکیده
Infectious diseases have become part of human civilization as a constant phenomenon throughout time. The Human Immunodeficiency Virus (HIV) is virus that can cause immune system weakness by attacking cells CD4 receptors and CCR5 or CXCR4 chemokine coreceptors, namely helper T macrophages dendritic cells. Therefore, people infected with this will be vulnerable to opportunistic infections cancer. Here we proposed the translational bioinformatics approach from dry wet bench. From proof concept applications highlighted discussed further in diagnostic HIV vaccine candidate development based on reverse vaccinology approach. We performed sequence data analysis find best epitope for strains Indonesia compatible MHC class 1, linear core 2, B cell epitope. Epitope two expected high immunogenicity potentially produce response good memory. was then tested using an indirect ELISA against serum positive patients. In study, negative control used blood healthy individuals originating Stored Biological Material carried out commercial kit controls. PBS buffer solution coated microplate incubation time 16 hours various concentrations. As result, concentration range 1-2500 ng epitope/well recognized patient antibodies. summary, silico prediction had antigenicity anti-HIV antibodies sera samples could also promising vaccine.
منابع مشابه
Translational Antidote Research: A Bedside to Bench Tale
Although antidote development should proceed in an orderly fashion from observation, to experimental and safety studies, to clinical trials, this sequence is not always precisely followed. The development of fomepizole as an antidote for toxic alcohol and glycol poisoning is an example of how this may not be the case. Interest in the development of fomepizole was spurred in the 1960s. Shortly t...
متن کاملtranslational antidote research: a bedside to bench tale
although antidote development should proceed in an orderly fashion from observation, to experimental and safety studies, to clinical trials, this sequence is not always precisely followed. the development of fomepizole as an antidote for toxic alcohol and glycol poisoning is an example of how this may not be the case. interest in the development of fomepizole was spurred in the 1960s. shortly t...
متن کاملIntroduction to Translational Bioinformatics Collection
How should we define translational bioinformatics? I had to answer this question unambiguously in March 2008 when I was asked to deliver a review of ‘‘recent progress in translational bioinformatics’’ at the American Medical Informatics Association’s Summit on Translational Bioinformatics. The lecture required me to define papers in the field, and then highlight exciting progress that occurred ...
متن کاملTranslational studies in neurorehabilitation: from bench to bedside.
Stroke is the leading cause of adult disability in the western world. Consensus has built over the last few years regarding the usefulness of training to improve motor disability resulting from stroke. Until recently, there were no accepted strategies to enhance the beneficial effects of training. However, the combination of basic and clinical science data over the last few years is changing th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BIO web of conferences
سال: 2021
ISSN: ['2273-1709', '2117-4458']
DOI: https://doi.org/10.1051/bioconf/20214102002